Higher Rates of Chromatography Production Using Convection & Purexa membranes
The straight-through pores present in membranes allow for purification to be completed through a convection dominated process. As an advantage of this design, membranes can operate at very short residence times. Additionally, with large biologics, there are minimal size exclusion effects on membrane applications. With a pore size generally between 100 nm and a few micron meters, the membrane can have a much higher binding capacity and flow rate for gene therapy products when compared to resins.
Resin columns usually require a very long residence time, hindering the productivity of biologics purification. This issue of inadequate productivity becomes more significant with larger gene products, including mRNA, plasmid DNA, AAV, and LV vectors.
Purexa™ chromatography membranes achieve high production volumes with rapid changeover and consistent performance
Purexa™ Membranes can produce faster flow rates with no incubation periods, all with easier set up and tear down, with consistency of production and longevity of results when compared to resins. This solution performs while it reduces production stressors and concerns
Higher Performance at Higher Speeds
Running with a 800 nt mRNA sample, Purexa™ OdT has more than twice the dynamic bonding capacity (DBC) while operating at 20x the flowrate compared to resins.
Purification can be completed in a matter of minutes, rather than hours!
Purexa™ MQ is a multimodal anion-exchange membrane. To determine the impact of flow rate on the binding capacity of Purexa™ MQ, we tested the DBC of the membrane using salmon sperm DNA (120,000 – 300,000 Dalton) and Bovine Serum Albumin (BSA; 60,000 Dalton) at 10 Membrane Volumes per minute (MV/min) and 50 MV/min. Results of this experiment can be noted in Figure 1: Purexa™ MQ DBC vs Flow Rate. Read More on Flow Rate & Binding Capacity here
Recent Purexa™ Case Studies
Purexa™ Membrane Form Factors
Purilogics offers a broad portfolio of Purexa™ membrane series products in various form factors. This linear scalability provides optionality to reach precise yield targets.
Custom Membrane Development
Due to large pore structure, membranes are well-suited for large molecule purification, particularly for gene & cell therapy products. Purilogics’ expert team offers specialized surface modifications, mixed-mode ligand development, as well as customized ligand and enzyme immobilizations. We can tailor modifications on membrane surfaces with various pore sizes and achieve specific ligand densities. Let us help with your membrane needs.